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Table 2 Identification of hnRNP C1/C2, H1, and H2 from all 2DE gel spots

From: Age-related expression analysis of mouse liver nuclear protein binding to 3′-untranslated region of Period2 gene

SSP number pIr Mr Spot quantity MS hit score Spot info MS hit score in the mixture
HnRNP C1/C2
 A2319 5.2402 37,099.7 518 64 Single 0
 A2328 5.0951 36,932.8 371.8 129 Mix 58
 A2427 5.0982 38,763.6 179.5 63 Single 0
 A2431 5.0805 37,737.2 886.2 239 Mix 44
 A2432 5.1581 37,737.2 1071.3 63 Single 0
 A2433 5.1672 38,817.7 265.2 57 Single 0
 A4328 5.7305 35,918.0 49.9 126 Mix 67
 B6819 9.1533 82,564.2 16.8 46 Single 0
 B8328 9.5825 35,397.8 0 49 Single 0
 B9112 10.0112 17,936.2 66.1 66 Single 0
HnRNP H1
 N4520 5.9448 53,089.3 53.5 119 Single 0
 N5502 5.9950 56,267.0 925.8 342 Mix 94
 N5523 6.3721 52,715.2 187.6 295 Mix 58
 N5525 6.0081 55,342.4 0 222 Mix 85
 N6405 6.4684 46,614.3 57.8 133 Single 0
 N6422 6.4560 48,411.4 210.7 274 Mix 60
 N6514 6.8543 56,426.1 142.1 182 Single 0
HnRNP H2
 A1520 4.9649 47,840.4 349.7 196 Single 0
 N3521 5.8593 56,011.9 200.2 298 Mix 119
 N4511 5.9372 55,463.9 2029.8 223 Mix 140
 N4526 5.9962 54,836.2 97.2 167 Mix 111
 N6407 6.5283 49,276.6 338.1 278 Mix 68
  1. All spots for samples from 3-month-old mice were identified by MALDI-TOF/MS PMF analysis (total number of spots, 3113; number of single spots, 2557; number of mixed spots, 556). Spots identified as hnRNP C1/C2, H1, and H2 are listed. A total of 10, 7, and 5 spots were identified as hnRNP C1/C2, hnRNP H1, and hnRNP H2, respectively, among all 2DE spots analyzed. The locations of these spots are shown in Fig. 2c, e and Figs. S6, S7, and S8