Fig. 1
RNA-EMSA of 32P-labeled mPer2 sl-RNA with mouse liver NE. a Purine–pyridine sequence in the 3′-UTR of the Per2 gene. b Location of the potential hairpin sequence in the 3′-UTR (starting at nucleotide 4006) of the mPer2 gene. c The potential 3′-UTR hairpin (starting at nucleotide 4006) in the mPer2 gene. d RNA-EMSA of the 32P-labeled mPer2 sl-RNA probe followed by UV cross-linking and RNase treatment. RNA-EMSA of 32P-labeled mPer2 sl-RNA with NE. Arrowheads indicate the mPer2 sl-RNA probe–protein complexes. Lanes 1–5 contain 32P-labeled mPer2 sl-RNA and RNase T1. Lane 1 no NE; lanes 2– 4 with liver NE. Asterisk indicates that band intensity did not increase with increasing amount of NE added. e Competitive RNA-EMSA of 32P-labeled mPer2 sl-RNA with NE. Lanes 1–5 contain 32P-labeled mPer2 sl-RNA and RNase T1. Lanes 2–4 contain unlabeled mPer2 sl-RNA and NE. Lane 5 contains no NE