Fig. 5

Effects of insulin on phosphorylation of ERK in A6 monolayered cells. a A6 monolayers were treated with 1 μM insulin for the indicated time, and then phosphorylation of ERK was detected by immunoblotting with an anti-phospho-ERK specific antibody. b Relative amounts of phosphorylated ERK are shown. Equal loading of cell lysate to each well was confirmed by measuring β-tubulin as an internal control. Data are presented as mean ± SEM. n = 3. * Significantly larger than that at 0 min (without insulin treatment) with p < 0.05. # Significantly smaller than that at 0 min (without insulin treatment) with p < 0.05