Fig. 5
From: Retardation of C2C12 myoblast cell proliferation by exposure to low-temperature atmospheric plasma
Plasma exposure did not affect cell differentiation. C2C12 cells at approx. 100 % confluence were cultured in differentiation medium, which was changed every 24 h. Cells were treated with plasma for 1 min at 3.5 W every 24 h. Cells were lysed at 0, 1, and 3 days of culture in differentiation medium (n = 4–6). Representative western blots showing myogenin, fast myosin heavy chain (MHC), and β-actin proteins in control (Cont; argon gas flow only) and plasma exposed (Plas) cells are shown in (A). The total protein content in recovered cells was measured by BCA protein assay (B). The expressions of myogenin (C) and MHC (D) were analyzed by densitometry for quantification. Values with different letters are significantly different (p < 0.05)