Fig. 2
From: Retardation of C2C12 myoblast cell proliferation by exposure to low-temperature atmospheric plasma
Plasma exposure reduced the cell proliferation. C2C12 cells were treated with plasma for 1 min at 3.5 W every 24 h. A Effect of plasma exposure on cell numbers per well. At the indicated day of 2 h after the plasma exposure, cells were recovered by 0.25 % trypsin-EDTA solution. Cells were stained with 0.2 % trypan blue/PBS for 10 min. Non-stained (viable) cells were counted under a microscope using a hemocytometer (n = 4–6 per group). B Total protein content in recovered cells per well was determined by a BCA protein assay. IC incubator control cells, Cont the cells were treated with argon gas flow without plasma, Plas the plasma-exposed cells, Med the culture medium of control cells were replaced with plasma-exposed medium. *Significantly different from IC, Cont, and Med groups on the same day (p < 0.05). Values with different letters are significantly different (p < 0.05)