Fig. 10
From: Retardation of C2C12 myoblast cell proliferation by exposure to low-temperature atmospheric plasma
Plasma exposure affected the expression of cell-cycle checkpoint proteins. Cells were treated with plasma exposure (3.5 W) for 1 min, and at 8 h after plasma exposure, the cells were lysed with RIPA lysis buffer and subjected to western blot analysis for phospho (P)-cdc2, cyclin B1, p21 Waf-1/Cip-1 (p21) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (A). Effect of plasma exposure on the expression of phospho-cdc2 (B), cycline B1 (C) and p21 Waf-1/Cip-1 S (D) were analyzed by densitometry for quantification (n = 4–6). *Significantly different from Cont (argon gas flow only) group (p < 0.05)