Fig. 3

Close location of mitochondria and the ER/SR in A20 B lymphocytes (a) and in HL-1 cardomyocytes (b). Cells were co-transfected with mitochondria-targeted pTagRFP-mito (red) and ER/SR-targeted cameleon D1ER (green). Images were acquired by use of a laser-scanning confocal microscope (LSM710, Carl Zeiss) with a ×63 oil objective lens, and 3D images were reconstructed by use of Imaris (Bitplane) (color figure online)