Fig. 3

Ferucarbotran/AMF-induced hyperthermia enhances the pro-apoptotic effect of cisplatin in human oral cancer cells. Annexin-V/PI staining of human oral cancer cells at 12-h intervals after treatment with 0, 7.5, or 15 μM cisplatin with or without hyperthermia (HT) in HSC-3 cells, and 0, 15, 30 μM cisplatin with or without HT in OSC-19 cells. a Representative analysis of apoptosis of HCS-3 cells and OSC-19 cells exposed to cisplatin and ferucarbotran with or without AMF. Annexin-V/PI method with FACS scan dot plot analysis was used to divide the treated and control cells into four groups: (1) living cells (lower left quadrant); (2) necrotic cells (upper left quadrant); (3) early apoptotic cells (lower right quadrant); and (4) late apoptotic cells (upper right quadrant). b Representative analysis of apoptosis of OSC-19 cells exposed to cisplatin with or without AMF. *p < 0.05, **p < 0.01; n = 4