Fig. 5

5-Hydroxytryptamine (5-HT)-containing enterochromaffin cells in the mouse cecal mucosa. Ten-μm-thick sections of the mouse cecum were stained by using the goat anti-5-HT antibody and Alexa594-labeled anti-goat IgG antibody as the secondary antibody (red). 4′,6-Diamidino-2-phenylindole (1 μg/mL) was used with the secondary antibody for staining the nuclei (blue). a Images of whole layers of the mouse cecal wall, including mucosa (Muc), submucosa (SM), circular muscle (CM) and longitudinal muscle (LM) were taken with a ×20 objective lens (bar 20 μm). b Magnified image of the rectangular region in image A taken with a ×40 objective lens. Arrowheads indicate the apical plasma membrane of the 5-HT-immunoreactive enterochromaffin cells accessing the lumen with a fine process (color figure online)