Fig. 1
Apical proton regulates intestinal passive Mg2+ transport. The relative cell viability of Caco-2 exposed to acidic apical culture medium, omeprazole, and omeprazole plus acidic was elucidated by MTT assay (a). Passive Mg2+ transport across Caco-2 monolayer was measured in the presence of acidic apical medium, omeprazole plus acidic apical medium (b), acidic apical bathing solution, and omeprazole plus acidic apical bathing solution (c). Representative time line of Caco-2 monolayer experiments (d). *P < 0.05, **P < 0.01, ***P < 0.001 compared with the corresponding pH 7.4 group. †† P < 0.01, ††† P < 0.001 compared with the omeprazole-free pH 7.4 group. (n = 6)