Fig. 3
Concentration-dependent effects of PGE2 and EP agonists on the induction of GCLCs. PGE2 and EP1 (ONO-DI-004), EP2 (ONO-AE1-259), EP3 (ONO-AE-248), and EP4 (ONO-AE1-329) agonists were cumulatively added to the bath solution of tissues pretreated with piroxicam. Representative traces showing the cumulative addition of PGE2 (a), ONO-DI-004 (EP1 agonist; b), ONO-AE1-259 (EP2 agonit, c), ONO-AE-248 (EP3 agonist; d), and ONO-AE1-329(EP4 agonist, e). Concentration–response curves for the effects of PGE2 and EP agonists on the frequency (f) and mean amplitude (g) of GCLCs. Values are mean ± SEM. The numbers in (g) indicate the number of preparations, excluding preparations with a mean amplitude of 0. The mean amplitude of tissue preparations treated with the EP2 and EP4 agonists is not shown