Fig. 8

Immunohistochemistry for SERCA2 in the bladder of WTBOO (a) and TGBOO (b). Photographs a and b are at ×20. a-a (indicated by small box in a) and b-b (indicated by small box in b) photographs are enlarged at ×400. The 0.5 (width) × 0.5 cm (height) sample of the posterior bladder wall was embedded in paraffin. Paraffin blocks were cross-sectioned in 5 μm thickness. The upper side is the mucosal layer. SERCA2 immunoreactivity was identified only in the cytoplasm of the smooth muscle cells (solid arrows in a-a and b-b), strongly positive in WTBOO (a-a) and TGBOO (b-b). SERCA2 immunoreactivity was negative in interstitial cells (open arrows in a-a and b-b) and the mucosal layer