Fig. 4

Azan stain and immunohistochemistry for SERCA2 in WT (a, c) and TG (b, d) bladders. Photographs a, b, c and d are at ×20. Photographs a-a (indicated by small box in a), b-b (indicated by small box in b), c-a (indicated by small box in c), and d-b (indicated by small box in d) are enlarged at ×400. The 0.5 (width) × 0.5 cm (height) sample of the posterior bladder wall was embedded in paraffin. Paraffin blocks were cross-sectioned in 5 μm thickness. The upper side is the mucosal layer. SERCA2 immunoreactivity was identified only in the cytoplasm of the smooth muscle cells (solid arrows in c-a and d-b), weakly positive in WT (c-a) and strongly positive in TG (d-b). SERCA2 immunoreactivity was negative in interstitial cells (open arrows in c-a and d-b) and the mucosal layer