Fig. 1
From: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway
Endothelial ABCG1 expression was correlated with cholesterol efflux and intracellular lipid content. a, b HUAEC were transfected with scrambled siRNA, ABCG1 siRNA and either pcDNA (empty vector) or a pReceiver-ABCG1 expression vector. ABCG1 mRNA (a) and protein expression (b) were measured by real time PCR and western blotting. c Cholesterol efflux to HDL was decreased in ABCG1-deficient EC (ABCG1−), and reversed in ABCG1 overexpression plasmid transfection (ABCG1+). d Intracellular cholesterol content was increased in ABCG1-deficient EC while decreased in ABCG1 overexpression plasmid transfection. TC total cholesterol, FC free cholesterol, CE cholesterol ester. Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.001 vs. EC control