Fig. 1

H₂O₂ facilitates L-type Ca²⁺ channel activity in cell-attached mode. a Time course of channel activity (NPo) recorded in the cell-attached mode before and after application of 1 mM H₂O₂. b Examples of current traces of the Ca²⁺ channels before (a) and after (b) application of H₂O₂ taken at the times indicated in (a). c Effect of 1 mM H₂O₂ on channel activity (NPo) recorded in cell-attached mode in the presence of 10 μM KN-93. d Summary of the normalized activity of the Ca²⁺ channel treated with H₂O₂ with no drug (n = 6), and with 10 μM KN-93 (a specific CaMKII inhibitor) (n = 9) or KN-92 (an inactive form of KN-93) (n = 5). Mean channel activity (60 traces) in each patch was normalized to the corresponding control value, averaged in the same group, and shown as mean ± SE. *P < 0.05 and **P < 0.01 versus control (Student’s t test), and ^# P < 0.05 and NS not significant versus H₂O₂ without drug (ANOVA and Tukey HSD test)