Fig. 4
From: Elevation of muscle temperature stimulates muscle glucose uptake in vivo and in vitro
Elevation of muscle temperature has direct impacts on muscle signal transduction pathways associated with glucose uptake. Isolated extensor digitorum longus muscles of rats weighing ~60 g were incubated without 2-deoxyglucose for 10 min at either 36 or 42 °C. Phosphorylation of signaling molecules was detected by immunoblot analysis with antibodies against p-AMPK (Thr172), p-Akt (Thr308), p-p38 MAPK (Thr180/Tyr182), p-TBC1D1 (Ser700), and p-TBC1D4 (Thr642). A representative blot is shown above each bar. Data are expressed as the mean ± SE (n = 4–8). # p < 0.05 as compared to the control (36 °C). Temp temperature