Fig. 3
From: Elevation of muscle temperature stimulates muscle glucose uptake in vivo and in vitro
Time course for glucose uptake in vitro in response to heat stimulation, cessation of stimulation, and re-stimulation in vitro. Incubation schedules for the time-course experiment are shown in a. Isolated extensor digitorum longus muscles of rats weighing ~60 g were pre-incubated without 2-deoxyglucose (2DG) for 20 min according to the schedules in incubation 1 (10 min at 36 °C + 10 min at 36 °C), incubation 2 (10 min at 36 °C + 10 min at 36 °C), incubation 3 (10 min at 36 °C + 10 min at 42 °C), incubation 4 (10 min at 42 °C + 10 min at 36 °C), or incubation 5 (10 min at 42 °C + 10 min at 36 °C). After the pre-incubation, muscles were then incubated with 2DG for 10 min at either 36 °C (incubations 1, 3, 4) or 42 °C (incubation 2, 5) (b). Data are expressed as the mean ± SE (n = 5–7). # p < 0.05 as compared to the control (incubation 1).¶ p < 0.05 as compared to the incubations 1, 3, and 4