Fig. 8

Effect of 2-APB on the IL-1β-induced suppression of channel activity in the Ca²⁺-free bath solution. a Representative current trace showing that the IL-1β suppressed channel activity in the presence of 2-APB with the Ca²⁺-free bath solution. b The effect of IL-1β in the presence of 2-APB was blocked by GF109203X. Filled bar above the trace indicates the use of Ca²⁺-free bath solution. The NP _o values calculated from these current traces were 0.77–0.82 for controls, 0.76–0.82 for 2-APB alone, 0.19 for 2-APB + IL-1β, 0.76 for 2-APB + GF109203X, and 0.81 for 2-APB + GF109203X + IL-1β. The doses of 2-APB, IL-1β, and GF109203X were 1 μM, 15 pg/ml, and 300 μM, respectively. Each trace was obtained from separate cell-attached patches at a V _p of 0 mV. c Summary of the effects of 2-APB (n = 17), IL-1β in the presence of 2-APB (n = 7), and IL-1β in the presence of 2-APB and GF109203X (n = 10). ** P < 0.01, significantly different compared with the initial control level