Fig. 6

Effect of a PLC inhibitor, neomycin, on the IL-1β-induced rise in [Ca²⁺] _i . a Representative trace of Fura-2 Ca²⁺ imaging which shows that neomycin blocked the IL-1β-induced rise in [Ca²⁺] _i . IL-1β was still capable of increasing [Ca²⁺] _i after washing out neomycin. The doses of neomycin and IL-1β were 300 μM and 15 pg/ml, respectively. b Summarized data showing [Ca²⁺] _i under the control condition (n = 9) and [Ca²⁺] _i in response to neomycin (n = 9), IL-1β in the presence of neomycin (n = 9), and IL-1β alone (n = 9). *P < 0.05, significantly different compared with the control