Fig. 4

Effect of a PLC inhibitor on the IL-1β-induced suppression of channel activity. a Representative current trace showing that IL-1β did not suppress channel activity in the presence of neomycin, whereas IL-1β alone suppressed the channel after washing out neomycin. In this trace, the calculated NP _o values were 0.78 for control, 0.82 for neomycin alone, 0.83 for neomycin + IL-1β, and 0.05 for IL-1β alone. The doses of neomycin and IL-1β were 300 μM and 15 pg/ml, respectively. b Summary of the effects of neomycin (n = 11), IL-1β in the presence of neomycin (n = 11), and IL-1β alone after washing-out (n = 7). **P < 0.01, significantly different compared with the initial control level