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Table 2 Fitting parameters for the bindings of CaM and CaM34 to NT

From: Lobe-related concentration- and Ca2+-dependent interactions of calmodulin with C- and N-terminal tails of the CaV1.2 channel

 

CaM

CaM34

EGTA

100 nM

10 μM

2 mM

EGTA

100 nM

10 μM

2 mM

Kd (μM)

0.10

0.02

5.00

0.77

0.12

0.04

1.24

0.98

Bmax (mol/mol)

0.02

0.02

0.20

0.33

0.02

0.02

0.16

0.27

R2 (one-site model)

0.910

0.982

0.952

0.983

0.957

0.988

0.922

0.977

  1. Data from the GST pull-down assay shown in Fig. 4 were analyzed with SigmaPlot 10.0 and with a single Hill equation (see “Materials and Methods”). Parameters are similar to those in Table 1
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The Journal of Physiological Sciences

ISSN: 1880-6562

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