Fig. 3

CaM₃₄ and CaM₁₂₃₄ binding to CT1 peptide of the Ca_V1.2 channel. GST pull-down assay for CaM₃₄ (a) or CaM₁₂₃₄ (c) binding to CT1. Protein bands are shown as CBB staining at different [Ca²⁺]. CaM bands are indicated by arrows. Plots of concentration-dependent binding with fitted curves of CaM₃₄ (b) and CaM₁₂₃₄ (d) (0.1–50 μM) to CT1 at ≈ free, 100 nM, 10 μM and 2 mM Ca²⁺. Band densities were converted into molar ratio (CaM₃₄/GST-CT1) and plotted against total [CaM₃₄] or [CaM₁₂₃₄] with symbols as indicated in each graph and with mean ± SE (n = 3–10)