Fig. 5

Effect of emulsified isoflurane on expression of Bcl-2 and Bax proteins visualized by Western blot analysis. Representative Western blot bands of Bcl-2 (26 kD), Bax (21kD) expression in the ischemic myocardium 3 h after reperfusion are shown. Corresponding GAPDH band as loading controls is shown in the upper panels. The box-plot graphs in the lower panel show quantification of the Western blot analysis for the respective proteins of Bcl-2 (a), Bax (b) and Bcl-2/Bax ratio (c) compared with GAPDH, respectively. Data are given as mean ± SD (n = 8, each group). *P < 0.05, **P < 0.01 vs sham; ^† P < 0.05, ^†† P < 0.01 vs CON; ^‡ P < 0.05, ^‡‡ P < 0.01 vs IL