Fig. 3
Caspase-3 protein expression and cleavage in myocardium of rats in each group. a Cleaved caspase-3 immunoreactivity in the cytoplasm of myocytes of hearts. Magnifications, 400×. b Western blot analysis of total and cleaved caspase-3 protein expression in hearts subjected to ischemia/reperfusion and receiving different treatments. Representative blots of total and cleaved caspase-3 (left). Data are quantified as percentage of cleaved caspase-3 of total caspase-3 and displayed using a box-plot. Data are presented as mean ± SD. Sham, Sham operated group; CON, control group; IL, 30 % intralipid group; EIso, emulsified isoflurane group of 2 ml kg−1. Each group n = 8. PEI, positive expressive index. *P < 0.05, **P < 0.01 vs sham; † P < 0.05, †† P < 0.01 vs CON; ‡‡ P < 0.01 vs IL