Fig. 2
Apoptosis determined by measurement of terminal transferase dUTP nick end labeling (TUNEL) positive cardiomyocyte nuclei in the area at risk of myocardium obtained from rats receiving saline (sham, CON), 30 % intralipid (IL) or 2 ml kg−1 emulsified isoflurane (EIso) during early reperfusion after coronary artery occlusion. Arrows denote TUNEL positive nucleus. Positive cells were not detected in non-ischemic zone after ischemia and reperfusion. Magnification 400×. Sham, Sham operated group; CON, control group; IL, 30 % intralipid group; EIso, emulsified isoflurane group of 2 ml kg−1. Box-plot graph illustrates the average percentage of TUNEL-positive ventricular myocytes in the ischemic regions of LVs. Each group n = 8. Data are expressed as mean ± SD. **P < 0.01 compared with CON