Fig. 2

Adenoviral infection of primary hepatocytes in culture alters bile salt cellular utilization. a Bile salts accumulated over 24-h periods in culture media. Infection was performed with 10 m.o.i. at 0 h and culture media were collected over 24-h periods, including the period prior to infection. Data represent the mean ± SE of nine samples from three different preparations. Bonferroni’s test: ^aaa P < 0.001 compared with the 0–24 h period. b Time-course analysis of bile salt uptake. 24 h after infection cells were incubated with a [¹⁴C]taurocholate-containing medium (50 μM, 3 Ci mol⁻¹) and intracellular radioactivity was measured at eight time points. Results from each experiment were normalized internally prior to being combined and are presented in arbitrary units (au); data represent the mean ± SE of nine samples from three different preparations. Two-way ANOVA analysis: P < 0.01 due to the treatments. Bonferroni’s test: *P < 0.05 compared with control cells. c Time-course analysis of bile salt secretion. 24 h after infection cells were incubated with the same medium as in b for 30 min, after which it was replaced with fresh medium and intracellular radioactivity was measured at eight time points. Data represent the mean ± SE of nine samples from three different preparations. Two-way ANOVA analysis: P < 0.001 due to the treatments. Bonferroni’s test: *P < 0.05, **P < 0.01, ***P < 0.001 compared with control cells; ^# P < 0.05 compared with AdSS-infected cells