Fig. 7
Effects of tachykininergic and cholinergic blockers on contractile responses elicited by high-frequency EFS in the hamster colon at 30 days after TNBS treatment. a A representative tracing showing the effects of L-732,138 (1 μM) and SR48968 (1 μM), blockers for tachykinin NK1 and tachykinin NK2 receptors, atropine (1 μM), an antagonist of muscarinic acetylcholine receptors, suramin (400 μM), an antagonist of purinocetors, naloxone (100 μM), an antagonist of opioid receptors (100 μM), and tetrodotoxin (1 μM), a blocker of voltage-dependent sodium channels on neurons on EFS-induced contraction, in the post-inflamed hamster colon. EFS was applied using multi-pulses of 20 V each, with a 0.3-ms pulse duration at 20 Hz for 1 s; longitudinal mechanical responses were recorded isometrically. Circles Points of electrical stimulations. b Summary graphs of the effects of L-732,138 (1 μM), SR48968 (1 μM), atropine (1 μM), suramin (400 μM), naloxone (100 μM) and tetrodotoxin (1 μM) on EFS-evoked contraction in the post-inflamed hamster colon (n = 10). Each value represents the mean ± SD. **p < 0.01 compared to the control, which is the response before addition of blockers