Fig. 6

Effect of an NOS inhibitor on relaxation responses and effects of tachykininergic and cholinergic blockers on contractile responses elicited by low-frequency EFS in the hamster colon at 30 days after TNBS treatment. Summary graphs of the effect of L-NAME (200 μM) on EFS-evoked relaxation (a) and effects of L-732,138 (1 μM), SR48968 (1 μM) and atropine (1 μM) on EFS-induced contraction in the presence of L-NAME (200 μM) (b) in the post-inflamed hamster colon (n = 5). EFS was applied using multi-pulses of 20 V each, with a 0.3-ms pulse duration at 2 Hz for 1 s; longitudinal mechanical responses were recorded isometrically. Each value represents the mean ± SD. **p < 0.01 and *p < 0.05 compared to the control, which is the response before addition of blockers