Detection of dihydropyridine- and voltage-sensitive intracellular Ca2+ signals in normal human parathyroid cells

Table 1 Effect of Ca²⁺ channel modulators on the Ca²⁺ transient induced by extracellular application of 150 mM K⁺ or 3 mM Ca²⁺

Stimulator^a	Modulator^b	Control	With modulator	No. of cells
150 mM K⁺	Nicardipine (1 µM)	2.68 ± 1.50	0.10 ± 0.04	15
	FPL-64176 (10 µM)	0.96 ± 0.90	6.91 ± 3.37	10
	Cd²⁺ (0.5 mM)	2.52 ± 0.93	0.03 ± 0.06	21
3 mM Ca²⁺	Nitrendipine (1 µM)	4.30 ± 1.23	0.19 ± 0.30	20
	Nicardipine (1 µM)	4.88 ± 1.72	0.10 ± 0.08	20
	Cd²⁺ (0.5 mM)	2.13 ± 1.67	0.02 ± 0.02	12

Data are presented as the mean ± standard deviation. In each row, data {maximal change in change in fluorescence in response to stimulation/baseline fluorescence (∆F/F ) from baseline fluo-3 fluorescence following 10 s exposure to 150 mM [K⁺]_o or 3 mM [Ca²⁺]_o solution} were obtained from the population of a single primary culture dish before and after exposure to the modulators
^a [Ca²⁺]_o in the bath solution was 2 mM, except in the case of FPL-64176, for which [Ca²⁺]_o was 1.2 mM to highlight the effect of the agent
^bFor FPL-64176 and Cd²⁺, data with a marked increase in baseline fluorescence were excluded from the table

ISSN: 1880-6562