Fig. 4

Overexpression of constitutively active AMPKα1 plasmid (caAMPKα1). a Schematic diagram of the wild-type and constitutive active AMPKα1 protein. b Tendon fibroblasts cells were transfected with either caAMPKα1 (2 or 4 μg/ml) or control vector. Whole cell lysates were examined for AMPK, caAMPKα1, and phosphorylation of caAMPKα1 by immunoblotting. Anti-AMPK antibody having an N-terminal epitope detected exogenous caAMPKα1 (approximately 35 kDa) and endogenous wild-type AMPK (approximately 62 kDa). Actin was used as an internal loading control