Fig. 1

Treatment with HGF activated AMPK signaling pathway in tendon fibroblasts. a Primary tendon fibroblasts were treated with three different concentrations of HGF (10, 20, and 40 ng/ml) and then the intracellular AMPK phosphorylation (p-AMPK to AMPK ratio) was determined by Western blotting analysis. Actin was used as a loading control. N = 6. *P < 0.05 versus CTRL (control). b The phosphorylation of ACC (p-ACC to ACC ratio), a critical downstream factor of AMPK, in HGF-treated tendon fibroblasts was also determined. N = 6. *P < 0.05 versus CTRL