Fig. 3

Beating ACMs detected in the culture of CMDF cells pre-exposed to ischemia. a The cell number of beating ACMs identified in culture of CMDF cells with or without (control) pre-exposure to ischemia. Closed symbols indicate individual values and open symbols the mean values (n = 8 cell preparations). **p < 0.001. Right panels indicate phase contrast microscopy of beating ACMs cultured for 6 days. Bar 50 µm. b Spontaneous Ca²⁺ transients recorded from fluo 4-loaded ACMs identified within culture of CMDF cells that were or were not (control) pre-exposed to ischemia. Representative traces show the fluorescent time course at the site marked by the arrow on the line-scan images. c The peak intervals of the spontaneous Ca²⁺ transients. Open circles indicate the individual values and closed circles the mean ± SEM (n = 22 cells for control, n = 15 cells for ischemia obtained from 3 mice). No statistically significant differences were noted