Fig. 2

5-HT immunolabeling intensity in the NTS. Representative digital photomicrographs of 5-HT immunolabeling in the cNTS. Line drawing shows cNTS (green shading; adapted from [28]. Top is 5-HT labeling in the cNTS of a rat treated with VEH; bottom is 5-HT labeling in the cNTS of a rat treated with PEG. For purposes of presentation, images were adjusted for brightness and contrast; however, for quantification of intensity, background was subtracted from the images, but no adjustments for brightness or contrast were made. Boundaries of the cNTS are indicated by dotted white lines; scale bars 100 μm; cc central canal; ts tractus solitarius; dmv dorsal motor nucleus of the vagus