Table 1 Specific primers used in the real-time RT-PCR
Gene | Forward primer (5′–3′) | Position | Reverse primer (5′–3′) | Position |
|---|---|---|---|---|
IGFBP-1 | TGCCGGAGTTCCTAACTGTTGT | 161 | CCAGCGACTACGCGAACCT | 233 |
IGFBP-2 | GTGATGCCTGGCCCACTTA | 113 | TCGTCTTGATTTCCTGGACAATG | 184 |
IGFBP-3 | TGCTGGGAGTGTGGAAAGC | 494 | GAGTGGATGGAACTTGGAATCAGT | 563 |
IGFBP-4 | GCATCCCAACAACAGCTTCA | 607 | ATCTCTCACTTTGGCCATATGCTT | 685 |
IGFBP-5 | TGTGTGGACAAGTATGGGATGAA | 1274 | AAGGCGTGGCACTGAAAGTC | 1344 |
IGFBP-6 | TCCCTGCTGGTGTGTGGAT | 593 | GAGAGCTTCCCTGGCCATCT | 660 |
IGF-I Ea | AGCTGAGATAGTGTTTCCCAAAGG | 116 | TTCCAAACGCGAAATGAATG | 184 |
MGF | TGACATGCCCAAGACTCAGAAGT | 408 | CCTTCTCCTTTGCAGCTTCCT | 477 |
MyoD | GCCCGGTCTGCACTCATG | 1193 | GAGTGTCATTTAGCTTCATTTTTGG | 1267 |
PCNA | TCCGAAGGCTTCGACACATAC | 307 | GGACATGCTGGTGAGGTTCA | 375 |
myogenin | TGGTACCCAGTGAATGCAACTC | 504 | GGACCAAACTCCAGTGCATTG | 576 |
p21 | GGCAGACCAGCCTAACAGATTT | 469 | GGCACTTCAGGGCTTTCTCTT | 542 |
GAPDH | AGACTGTGGATGGCCCCTCT | 622 | GATGACCTTGCCCACAGCCT | 728 |