Fig. 4

Effects of neonatal estrogenization on Kiss1 mRNA expression in adult female rats. a Schematic representation of the experimental schedule of the experiment; EB estradiol benzoate, GDX gonadectomized, PND postnatal day, 8W 8 weeks, 9W 9 weeks. b Representative photographs of Kiss1 mRNA expressing neurons in ARC of 9W female rats that received oil or EB injection on PND0 and were GDX at 8W. 3V third ventricle. Scale bar 100 μm. c Mean number of Kiss1 mRNA expressing neurons in ARC. d Representative photographs of Kiss1 mRNA expressing neurons in AVPV/PeN. 3V third ventricle. Scale bar 100 μm. e Mean number of Kiss1 mRNA expressing neurons in AVPV/PeN. Asterisks indicate significant differences between oil and EB groups (p < 0.05)