Fig. 7
Ca2+ release from the SR induced by the solution for rapid cooling at 3°C. After Ca2+ loading of the SR, the temperature of the solution for the wash (G1R, Table 1) and the pre-assay solution (G0RM1.5F, Table 1) was gradually lowered from 24 to 3°C, and then the assay solution that contained ATP and fluo-3 (G0M1.5F) was applied at 3°C to the pre-cooled preparation as indicated at the top. Fluo-3 fluorescence signals were measured and were calibrated in terms of [Fluo-3-Ca]. An optical shutter was closed at a time indicated by an arrow