Fig. 2

Effects of IPC and chelerythrine on cytosolic cytochrome c protein expression (a), caspase-3 activity (b), and TUNEL-stained histology of rat liver (c). Cytosolic cytochrome c protein expression in the liver was measured by immunoblots at 3 and 24 h after reperfusion. Caspase-3 activity in the liver was measured at 3 and 24 h after reperfusion and the level of the sham group was arbitrarily set at 100.0%. TUNEL-stained histology of rat liver was measured at 24 h after reperfusion (×400). Results are presented as mean ± SEM of sham, 3 h (n = 8); I/R, 3 h (n = 9); IPC + I/R, 3 h (n = 10); CHE + IPC + I/R, 3 h (n = 10); sham, 24 h (n = 8); I/R, 24 h (n = 9); IPC + I/R, 24 h (n = 10); CHE + IPC + I/R, 24 h (n = 10). *,**Significant differences (P < 0.05, P < 0.01) versus the sham group; ⁺significant differences (P < 0.05) versus the I/R group; ^#,##significant differences (P < 0.05, P < 0.01) versus the IPC + I/R group