Fig. 6

Effect of the protease inhibitor cocktail (P8340; Sigma) on the I _sc increase induced by trypsin. The protease inhibitor cocktail was added to the serosal solution (diluted 1,000-fold) 20 min before adding 100 nM trypsin to the serosal solution. Peak values for the increases in I _sc (ΔI _sc) induced by trypsin were determined and are expressed as a percentage of the control response obtained in the adjacent tissue. Each data value is presented as the mean ± SE, with n = 4. ***P < 0.001, compared with the control response that had been determined in the adjacent tissue by a paired t test