Fig. 2

Effects of chloroquine on Kv1.3-channel currents in murine thymocytes in 500-ms single pulse protocols. Effects of 10 µM chloroquine at the voltgage steps of +40 (A), −40 (B) and −70 mV (C). a Typical whole-cell current traces recorded before and after the drug application, and after its washout. The currents were elicited from the holding potential of −80 mV to either +40 (A), −40 (B), or −70 mV (C) as depicted in the voltage protocols. Single pulses were applied for 500-ms durations between 1-min intervals. b Percentages of the pulse-end currents normalized by the peak currents obtained from the records in a. c Peak current densities (peak currents normalized by the membrane capacitance). ^# P < 0.05 versus before chloroquine application. Values are mean ± SEM (n = 6). Differences were analyzed by ANOVA followed by Dunnett’s or Student’s t test