Table 1 Forward/reverse primers and PCR conditions for ACE, ACTN3, and PPARD genotyping
Gene | Forward primer | Reverse primer | PCR reaction conditions | ||
|---|---|---|---|---|---|
(5′–3′) | (5′–3′) | Denaturation | Annealing and cycles | Final extension | |
ACE | CTGGAGACCACTCCCATCCTTTCT | GATGTGGCCATCACATTCGTCAGAT | 95°C for 5 min | 35 cycles of 95°C for 1 min, 58°C for 30 s, 72°C for 40 s | 72°C for 10 min |
ACTN3 (exon 16) | CTGTTGCCTGTGGTAAGTGGG | TGGTCACAGTATGCAGGAGGG | 95°C for 5 min | 35 cycles of 95°C for 1 min, 58°C for 30 s, 72°C for 40 s | 72°C for 10 min |
PPARD (exon 4) | CATGGTATAGCACTGCAGGAA | CTTCCTCCTGTGGCTGCTC | 95°C for 5 min | 35 cycles of 95°C for 1 min, 60°C for 30 s, 72°C for 40 s | 72°C for 10 min |