Table 1 Oligonucleotide primers used in RT-PCR
Gene name | GenBank Accession No. | Forward primer | Reverse primer | Expected size (bp) |
|---|---|---|---|---|
Heat shock protein 70 (Hsp70) | NM_031165 | 5′-AAATCATCAGCTGGCTGGATAAGAA | 5′-CATGCCACCTGCACTCTGGTA | 256 |
Proliferating cell nuclear antigen (Pcna) | NM_011045 | 5′-GTAGTTGTCGCTGTAGGC | 5′-GTTGCTCCACATCTAAGTC | 492 |
B-cell leukemia/lymphoma 2 (Bcl2) | NM_177410 | 5′-CCGCCTCTTCACCTTTCA | 5′-GGTTATCATACCCTGTTCTC | 348 |
Bcl2-associated X protein (Bax) | NM_007527 | 5′-CCAGGATGCGTCCACCAA | 5′-AAAGTAGAAGAGGGCAACCAC | 196 |
Cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2) | NM_009993 | 5′-CATCGGCTCCACTCCTGT | 5′-GCATCTCCTCGCTCTTCC | 413 |
Cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1) | NM_021282 | 5′-CCACCCTCCTCCTCGTAT | 5′-CTTGACAGCCTTGTAGCC | 218 |
Beta-actin (Actb) | NM_007393 | 5′-CTGTCCCTGTATGCCTCTG | 5′-ATGTCACGCACGATTTCC | 218 |