Fig. 4
Photomicrographs of results of TUNEL assay of liver sections prepared from the control mice and the mice exposed to 40, 42, 44, and 46°C whole body heat stress. The experiment was repeated three times and the representative pictures obtained by a digital image-capture system are shown. The arrowheads indicate the position of the apoptotic cells in the liver of unstressed control mice (a), 40°C stressed mice (b), 42°C stressed mice (c), 44°C stressed mice (d) and 46°C stressed mice (e). f Percentages of TUNEL-positive cells among total hepatocytes; at least 12-mm2 tissue sections were counted for each mouse. C unstressed controls. Significance: **P < 0.01 compared with controls. Scale bar 50 μm