Fig. 2

a Semi-quantitative RT-PCR of Hsp70, Pcna, Bax, Bcl2, CYP1A2 and CYP2E1 in the liver of control mice and the mice exposed to 40, 42, 44, and 46°C whole body heat stress. The experiment was repeated three times and the representative scan obtained by PhosphorImaging is shown. C unstressed control mice. Data obtained after quantification of amplification bands by Gel Pro Analyzer software 4.0. The values represent the mean ± SEM from individual animals presented on the above image. The intensities of the bands were normalized against the loading control of beta-actin. Fold increase of Hsp70 (b), Pcna (c), Bax (d), Bcl2 (e), CYP1A2 (f) and CYP2E1 (g) in the liver of mice after heat stress at different temperature was compared with the controls (the liver of unstressed mice). Significance: *P < 0.05, **P < 0.01 compared with controls