Fig. 1

PECAM-1-related trans-cellular migration at peri-junctional sites. During trans-cellular migration at a peri-junctional site, endothelial PECAM-1 was observed around the transmigrating monocyte. In this case, the PECAM-1 signal was seen at the lower left-hand edge of the transmigrating monocyte (arrows at 24 min, apical and basal including xz and yz images). Red monocytes. Green PECAM-1-GFP in ECs. The location of endothelial cell borders was delineated as curved white lines at identical locations on both the apical and basal sections at 24 min. Time after monocyte addition in minutes is shown in the upper-left corner. Maximum-projected xy-images toward the z-axis are shown, with xz/yz orthogonal images cut with the specified planes that are indicated as white lines on xy-images. At 24 min, z-stacks were optically separated into “apical” and “basal” sections. Bar 20 μm