Fig. 4

Effects of 3 days of hindlimb immobilization on total FOXO3a (total-FOXO3a; a) and phosphorylated FOXO3a (phospho-FOXO3a; b) protein expression, on the ratio of phospho-FOXO3a to total-FOXO3a protein levels (c), and on the percentage differences in this ratio from the control group (d) in soleus (SOL) and plantaris (PLA) muscles. The data are shown as relative values [to those of SOL muscle in the control (Con) group]. Each bar represents the mean ± SE (n = 3/group). Differences in protein expression between control muscles and those subjected to 3 days of immobilization were assessed by the Mann–Whitney U test. Significant differences from those of the control group for each muscle: SOL ^† P < 0.05; PLA ^# P < 0.05. Differences in percentage change of the ratio of phospho-FOXO3a to total-FOXO3a protein levels between soleus and plantaris after 3 days of immobilization were assessed by the Mann–Whitney U test. Asterisk (*) indicates significant difference between (P < 0.05) soleus and plantaris muscle after 3 days of hindlimb immobilization