Table 2 Sequence of the specific primers used in the quantitative PCR analyses
From: Effects of alfacalcidol on circulating cytokines and growth factors in rat skeletal muscle
Target mRNA | PCR primer sequence 5’ → 3’ | T m | Efficiency | R 2 | Acession number |
|---|---|---|---|---|---|
18S RNA | Forward: CGAACGTCTGCCCTATCAACTT Reverse: ACCCGTGGTCACCATGGTA | 77.27 | 102.0 | 0.998 | EU139318.1 |
α-skeletal actin | Forward: CGACATCGACATCAGGAAGGA Reverse: GGTAGTGCCCCCTGACATGA | 76.37 | 94.5 | 1 | NM_019212.2 |
IGF-1 Ea | Forward: AAGCCTACAAAGTCAGCTCG Reverse: TCAAGTGTACTTCCTTCTGAGTC | 78.76 | 91.3 | 0.997 | NM_178866.4 |
MAFbx | Forward: TGAAGACCGGCTACTGTGGAA Reverse: CGGATCTGCCGCTCTGA | 75.46 | 95.5 | 0.999 | NM_133521.1 |
MuRF-1 | Forward: TGCCCCCTTACAAAGCATCTT Reverse: CAGCATGGAGATGCAATTGC | 73.38 | 98.17 | 0.998 | NM_080903.1 |
Myostatin | Forward: GGTCCCGGAGAGACTTTGG Reverse: CGACAGCACCGCGATTC | 77.71 | 96.2 | 0.999 | NM_019151.1 |
c-myc | Forward: CACAACGTCTTGGAACGTCAGA Reverse: GCGCAGGGCAAAAAAGC | 75.34 | 91.3 | 0.998 | NM_012603.2 |
VDR | Forward: CACCCTTGGGCTCTACTCAC Reverse: CTGTTGCCTCCATCCCTGAA | 74.74 | 112.7 | 0.98 | NM_017058.1 |
CYP27B1 | Forward: CGGGAAAAGGTGTCTGTCCA Reverse: GTGTCCACTCCAGTAG | 75.48 | 107.2 | 0.966 | NM_053763.1 |