Table 1 Rattus norvegicus oligonucleotide sequences used in qRT-PCR experiments

Vitamin D-related genes

 VDR

NM_017058

5′-GACCTTGTCAGTTACAGCATC-3′

5′-AGACTGGTTGGAGCGTAA-3′

Transcellular genes

 TRPV5

NM_053787

5′-CTTACGGGTTGAACACCACCA-3′

5′-TTGCAGAACCACAGAGCCTCTA-3′

 TRPV6

NM_053686

5′-ATCCGCCGCTATGCAC-3′

5′-AGTTTTTCTGGTCACTGTTTTTGG-3′

 Calbindin-D_9k

X_16635

5′-CCCGAAGAAATGAAGAGCATTTT-3′

5′-TTCTCCATCACCGTTCTTATCCA-3′

 NCX1

NM_019268

5′-GTTGTGTTCGCTTGGGTTGC-3′

5′-CGTGGGAGTTGACTACTTTC-3′

 PMCA_1b

NM_053311

5′-CGCCATCTTCTGCACAATT-3′

5′-CAGCCATTGTTCTATTGAAAGTTC-3′

Paracellular genes

 NKA

X_63375

5′-CCACTGCTGAGCAGACACCAT-3′

5′-CCGAGTTCCAGATGAATTTCTTC-3′

 ZO-1

XM_218747

5′-GTATCCGATTGTTGTGTTCC-3′

5′-TCACTTGTAGCACCATCCGC-3′

 ZO-2

NM_053773

5′-TCTGAAGGTGAACACACAA-3′

5′-CCAGGATGTCTCTATACACG-3′

 ZO-3

XM_001069839

5′-TGCTAATGAGACCGCTAAAG-3′

5′-GACACTCCGTTGATCTGTAA-3′

 Cingulin

XM_001059265

5′-ATCGGGAACTCCAGTCAAC-3′

5′-TGACGGGAACGGCTAAAG-3′

 Occludin

NM_031329

5′-CACGTTCGACCAATGC-3′

5′-CCCGTTCCATAGGCTC-3′

 Claudin-2

NM_001106846

5′-GCTGCTGAGGGTAGAATGA-3′

5′-GCTCGCTTGATAAGTGTCC-3′

 Claudin-3

NM_031700

5′-GCACCCACCAAGATCCTCTA-3′

5′-AGGCTGTCTGTCCTCTTCCA-3′

 Claudin-12

XM_001067932

5′-CCTTCAAGTCTTCGGTGCC-3′

5′-CAGGAGGATGGGAGTACAG-3′

Housekeeping gene

 GAPDH

NM_017008

5′-AGTCTACTGGCGTCTTCAC-3′

5′-TCATATTTCTCGTGGTTCAC-3′