Fig. 6

a Typical staining patterns of HSC70 in the particular portions of the rat diencephalon. Immunoreacted signals of HSC70 were amplified by proximity ligation assay (PLA, red spots). Nucleus (blue), which indicates a single cell, was also labeled by Hoechst33342. Note that the red signals, which were detected by PLA, were generally observed in the cell bodies and the extracellular space in which nerve fibers and interstitial space were thought to exist. b The magnified images of HSC70-positive (blue arrowheads) and HSC70-negative (yellow arrowheads) cells in the medial and lateral regions of the diencephalon. The areas enclosed by the dotted circles indicate the extracellular space of the diencephalon, in which the PLA signals are also present. Pictures also show the higher intensity of the PLA signals in the extracellular space of the lateral region. CM Central medial thalamic nucleus, ic internal capsule, MDC central mediodorsal thalamic nucleus, MH medial habenular nucleus, mt mammillothalamic tract, PVP post paraventricular thalamic nucleus, VM ventromedial thalamic nucleus, VMH ventromedial hypothalamic nucleus, VPL ventral posterolateral thalamic nucleus, and VPM ventral posteromedial thalamic nucleus. Scale bars 50 μm (a) and 10 μm (b)