Fig. 5
From: Responses of HSC70 expression in diencephalon to iron deficiency anemia in rats
Western blot analysis of HSC70 expression in the crude extract of diencephalon. a Typical blot patterns of HSC70 (upper) and α-tubulin (internal control, lower). b Mean integrated areas of HSC70 bands. c Spots detected by Western blot using anti-HSC70 antibody following two-dimensional electrophoresis in the crude extract. Five spots (a–e) with different isoelectric points were detected in both the 100 ppm (upper) and 3 ppm (lower) Fe groups. d Mean percent integrated area (density × area) of each spot, relative to the whole five spots (a–e), in the 100 ppm (open bars) and 3 ppm (solid bars) Fe groups. Mean ± SEM. *p < 0.05 versus 100 ppm Fe group