Fig. 4

a Mass spectrum of the peptide fragments analyzed in the spots enclosed by dotted circles in Fig. 3b. b List of peaks seen in the spectrum. Thirty-nine peaks were detected. The peaks indicated by red characters were matched with the protein sequences listed in c. c Results of the NCBI database search. The distributions (numbers) of the scores of 44 proteins, shown by the three bars on the left side, were not significant. But those of six proteins with scores higher than 60 were significant. The names of those proteins are listed on the right. Five (nos. 1–4 and 6) out of six proteins were identified as heat shock protein 8 (70 kDa heat shock cognate protein, HSC70). d Full amino acid sequence of HSC70. The sequences estimated by the peptide mass, which are also indicated in b, are shown as red characters. A total of 34.5% of the full sequence of HSC70 was detected by the peptide mass, shown in red in b